external quality control (qc) Search Results


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Chem Impex International dss
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Cambridge Isotope Laboratories metabolomics quality control (qc) kit
Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted <t>metabolomics</t> analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).
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Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted <t>metabolomics</t> analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).
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Metronomia Clinical Research GmbH internal quality control (qc)
Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted <t>metabolomics</t> analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).
Internal Quality Control (Qc), supplied by Metronomia Clinical Research GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted <t>metabolomics</t> analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).
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SomaLogic assay data quality control (qc) procedures
Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted <t>metabolomics</t> analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).
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National Institute of Standards and Technology pooled quality control (qc) samples
Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted <t>metabolomics</t> analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).
Pooled Quality Control (Qc) Samples, supplied by National Institute of Standards and Technology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted metabolomics analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).

Journal: Cancer Immunology Research

Article Title: TCR-Independent Metabolic Reprogramming Precedes Lymphoma-Driven Changes in T-cell Fate

doi: 10.1158/2326-6066.CIR-21-0813

Figure Lengend Snippet: Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted metabolomics analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).

Article Snippet: The Metabolomics Quality Control (QC) Kit, which contains 14 stable isotope-labeled metabolite standards (Cambridge Isotope Labs), included the following compounds: L-alanine ( 13 C3, 99% purity), L-leucine ( 13 C6, 99%), L-phenylalanine ( 13 C6, 99%), L-tryptophan ( 13 C11, 99%), L-tyrosine ( 13 C6, 99%), caffeine ( 13 C3, 99%), D-glucose ( 13 C6, 99%), benzoate ( 13 C6, 99%), citrate ( 13 C3, 99%), octanoate ( 13 C8, 99%), propionate ( 13 C3, 99%), stearic acid ( 13 C18, 98%), succinic acid ( 13 C4, 99%), and D-sucrose ( 13 C6, 98%).

Techniques: Biomarker Discovery, Control, Flow Cytometry, Derivative Assay

Metabolic reprogramming of CD4 + T cells occurs early during Eμ- Myc lymphoma progression. A, Glu-Cy5 uptake capacity in CD4 + T cells from Eμ- Myc lymphoma-bearing C57BL/6J mice at the indicated timepoints, labeled ex vivo ( n = 5–10 mice). C, Control; E, early; L, late. B, Glu-Cy5 uptake capacity in CD45.2 + CD4 + T cells transferred into normal or Eμ- Myc lymphoma-bearing CD45.1 + congenic mice as in ( n = 5 mice). C, PCA based on untargeted metabolomics of isolated CD4 + T cells from Eμ- Myc lymphoma-bearing C57BL/6J mice at the indicated timepoints ( n = 6–7 mice). D, Heatmap of 59 metabolites that were differentially abundant by ANOVA at the indicated timepoints. E, Levels of select metabolites from glycolysis, glutaminolysis, the TCA cycle, and amino acid metabolism pathways that feed into the TCA cycle; G-6-P, glucose-6-phosphate; DHAP, dihydroxyacetone phosphate; 2/3-PGA, 2/3-phosphoglyceric acid; αKG, alpha-ketoglutarate. Data in A were analyzed by a one-way ANOVA with a Dunnett multiple comparisons; data in B were analyzed by a t test. Metabolomics data were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001 in A and B . *, FDR < 0.05 in E ; n.s., not significant).

Journal: Cancer Immunology Research

Article Title: TCR-Independent Metabolic Reprogramming Precedes Lymphoma-Driven Changes in T-cell Fate

doi: 10.1158/2326-6066.CIR-21-0813

Figure Lengend Snippet: Metabolic reprogramming of CD4 + T cells occurs early during Eμ- Myc lymphoma progression. A, Glu-Cy5 uptake capacity in CD4 + T cells from Eμ- Myc lymphoma-bearing C57BL/6J mice at the indicated timepoints, labeled ex vivo ( n = 5–10 mice). C, Control; E, early; L, late. B, Glu-Cy5 uptake capacity in CD45.2 + CD4 + T cells transferred into normal or Eμ- Myc lymphoma-bearing CD45.1 + congenic mice as in ( n = 5 mice). C, PCA based on untargeted metabolomics of isolated CD4 + T cells from Eμ- Myc lymphoma-bearing C57BL/6J mice at the indicated timepoints ( n = 6–7 mice). D, Heatmap of 59 metabolites that were differentially abundant by ANOVA at the indicated timepoints. E, Levels of select metabolites from glycolysis, glutaminolysis, the TCA cycle, and amino acid metabolism pathways that feed into the TCA cycle; G-6-P, glucose-6-phosphate; DHAP, dihydroxyacetone phosphate; 2/3-PGA, 2/3-phosphoglyceric acid; αKG, alpha-ketoglutarate. Data in A were analyzed by a one-way ANOVA with a Dunnett multiple comparisons; data in B were analyzed by a t test. Metabolomics data were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001 in A and B . *, FDR < 0.05 in E ; n.s., not significant).

Article Snippet: The Metabolomics Quality Control (QC) Kit, which contains 14 stable isotope-labeled metabolite standards (Cambridge Isotope Labs), included the following compounds: L-alanine ( 13 C3, 99% purity), L-leucine ( 13 C6, 99%), L-phenylalanine ( 13 C6, 99%), L-tryptophan ( 13 C11, 99%), L-tyrosine ( 13 C6, 99%), caffeine ( 13 C3, 99%), D-glucose ( 13 C6, 99%), benzoate ( 13 C6, 99%), citrate ( 13 C3, 99%), octanoate ( 13 C8, 99%), propionate ( 13 C3, 99%), stearic acid ( 13 C18, 98%), succinic acid ( 13 C4, 99%), and D-sucrose ( 13 C6, 98%).

Techniques: Labeling, Ex Vivo, Control, Isolation